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Description / Abstract
Bacteriophage lambda procapsids have shown promising uses as viral nanoparticles, as its structure allows for the possibility of drug encapsulation. The icosahedral procapsid consists of several individual proteins: the major capsid protein - gpE, head decoration protein - gpD, scaffolding protein - gpNu3, portal protein - gpB, and protease - gpC. In previous research, the in vitro assembly of the procapsids was attempted using only gpE, gpD, and gpNu3, with success in aggregation but not uniformity. The purpose of this project is to determine the effect of the presence of gpB and gpC on assembly, by cloning these proteins to have a glutathione-S-transferase (GST) affinity tag. After purification and incorporation of gpB and gpC, uniformity of procapsid formation improves.
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Download as a PDF: Honors Paper
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KeywordsBacteriophage Lambda, Procapsid Assembly, Viral Nanoparticles, Targeted Drug Delivery, Cloning, GST-affinity chromatography
Degree AwardedB.S. in Biology-Chemistry & B.A. in Spanish (Bethany Arabe), B.S. in Biology (Madelyn Stueve), B.S. in Biology (Tobias Wakefield-Carl)
Awarding InstitutionPoint Loma Nazarene University
Committee MembersKristopher Koudelka, Ariane Jansma, Michael Dorrell
Periodical TitleArete: The PLNU Honors Journal
Peer Reviewed?No
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PLNU Undergraduate Honors Projects 2021-2025
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